Description
This product uses the double-antibody sandwich method to detect T7 RNA polymerase in samples, which involves coating microplate wells with a monoclonal antibody specific to T7 RNA polymerase, and adding the standard and the test sample to the reaction well for incubation. T7 RNA Polymerase in the test sample will quantitatively bind to the antibody in the microplate, and the test procedure is to remove the unbound complex by washing, add the antibody against T7 RNA Polymerase (detection antibody), and finally add H + L secondary antibody (enzyme conjugate) to form an antibody-antigenantibody- secondary antibody complex, and indicate the protein content in the sample by observing the intensity of TMB color development. Please read the instructions for use carefully and check the components of the kit before use